Prof. Rana Pratap Singh Profile

Prof. Rana Pratap Singh

Adjunct Professor,

SCHOOL OF LIFE SCIENCES

E-mail: rana_singh[@]mail.jnu.ac.in , ranaps[@]hotmail.com

Phone: 91-11-26704503

Fax: 91-11-26742558

Education:

  • Ph.D in Cancer Biology, Jawaharlal Nehru University, New Delhi, India.
  • M.Sc. in Life Sciences, Jawaharlal Nehru University, New Delhi, India.
  • B.Sc. in Zoology, Botany, Chemistry. Ewing Christian College, University of Allahabad,U.P., India.

Career

  • 2012: Professor of Cancer Biology, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
  • 2012: Adjunct Professor, School of Life Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India.
  • 2010-2012:Professor and Dean, School of Life Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India. (Deputation)
  • 2006-2010: Associate Professor, Molecular Cancer Biology, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
  • 2003-2006: Assistant Professor - Research, Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Denver, CO, USA.
  • 2001-2003: Research Associate, Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Denver and Health Sciences Center, Denver, CO, USA.
  • 2000-2001: Post-doctoral Research Fellow, Center for Cancer Causation and Prevention, AMC Cancer Research Center, Denver, CO, USA.
  • Visiting Faculty (Research) at University of Colorado Denver, Aurora, USA
  • Visited Canada, China, Turkey and Austria for academic pursuits.

Research Interest

Cancer Chemoprevention, Tumor Angiogenesis

The areas of interest in carcinogenesis are cell cycle progression, mitogenic and cell survival signaling, and apoptosis, tumor angiogenesis, DNA damage/repair and organ specific carcinogenesis, including that of lung, prostate, etc. Discovering and evaluating anticancer activities of small molecules (phytochemicals) and providing scientific basis (mechanisms) for their effectiveness in controlling carcinogenesis is a priority area of research. Of specific importance is the understanding of mechanisms at all levels viz., molecular, cellular and organ levels in both in vitro as well as in vivo systems by using latest techniques. The goal is to develop mechanism-based non-toxic anticancer agents for their potential use in cancer chemoprevention and treatment.

Workshop

Conducted one-day workshop on "Cancer Biology and Basics of Genetic Engineering" at Gujarat Science City, Ahmedabad at the meeting of German Foundation "DWIH New Delhi - Excellence on Tour-2013". (November 15-22, 2013)

Organization of International Conferences/Symposium

  • Organized, International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (19-20 November 2013)
  • Organized, International Conference and Second Annual Meeting of Society of Mitochondria Research and Medicine-India on "Mitochondria in Health and Disease" at Central University of Gujarat, Gandhinagar, India. (2-3 November 2012)
  • Organized, International Conference on Recent Advances in Cancer Research: Therapeutics to Chemoprevention at Central University of Gujarat, Gandhinagar, India. (8-9 February 2012)
  • Organized, International Conference on Recent Advances in Cancer Research: Bench to Bedside at Central University of Gujarat, Gandhinagar, India. (19-20 February 2011)
  • Joint Organizer, International Cancer Research Symposium-2010 on Defining and Translating Science Behind the Disease at Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India. (20-22 December 2010)
  • Organized, International Symposium on Cancer Chemoprevention and Translational Research at Jawaharlal Nehru University, New Delhi, India. (21 December 2009)
  • Joint Coordinator, International Conference on Frontiers in Diagnosis, Prevention and Therapy of Cancer, Motilal Nehru Medical College, Allahabad, U.P., India. (21-22 November 2009)
  • Organized, International Symposium on Novel Strategies for Targeted Prevention and Treatment of Cancer at Jawaharlal Nehru University, New Delhi, India. (19-20 December 2008)

Awards/Honors

  • ICMR International Fellowship for Young Biomedical Scientists-2013-14. (University of California Riverside, USA)
  • Post-doctoral Trainee Award for Prostate Cancer Research, U.S. Army Medical Research and Materiel Command, Department of Defense, USA. (2003, for 2 years)
  • Scholar-in-Training Award, American Association for Cancer Research, Philadelphia, PA, USA. (2002)
  • JRF and SRF, University Grant Commission/School of Life Sciences, JNU, New Delhi, India. (1993-95-98)
  • National Eligibilty Test for Junior Research Fellowship and Lecturership, UGC/CSIR, New Delhi, India. (1993)
  • All India JNU Entrance examination for M.Phil./Ph.D. admission in Life Sciences, JNU, New Delhi, India. (First Rank, 1993).
  • Merit-cum-Means Scholarship for Masters, School of Life Sciences, JNU, New Delhi, India. (1991-1993)

Other/Administrative Activities

  • Scientific Advisory Committee Member, Institute of Cytology and Preventive Oncology, ICMR Institute, Noida, UP, India. (2013-)
  • Academic Council Member, Chhatrapati Shahu Ji Maharaj University, Kanpur, UP, India (2012-)
  • Dean of Students' Welfare, Central University of Gujarat, Gandhinagar, Gujarat, India. (2011-2012)
  • Dean, School of Life Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India. (2010-2012)
  • Moderation Committee Member , Gujarat State Eligibility Test, Gujarat, India. (2011, 2012)
  • Provost, Central University of Gujarat, Gandhinagar, Gujarat, India. (2010-2011)
  • Warden (Senior/Mess/Health/Recreation), Sabarmati Hostel, JNU, New Delhi. (2007-2010)
  • Chair, Search Committees, Project Research Assistant/Post-doctoral Fellows /Project Research Associate - Biofractionation, isolation, purification and characterization of active components having anticancer activity in natural extracts, and Cancer Intervention Research at DOPS/SOP/University of Colorado. (September 2005, and March 2006)
  • Invited Judge for 17th and 18th Annual Student Research Forum, University Colorado Health Sciences Center, Denver, CO, USA. (2002 and 2003)
  • Invited Judge for Denver Metropolitan Regional Science Fair, Denver, CO, USA. (2003)

Invited Talks

  • Department of Pharmaceutical Sciences, University of Colorado Denver, CO, USA. Title: "A novel role of silibinin in enhancing radiotherapeutic response in prostate cancer" (May 12, 2014).
  • University of California Riverside, Department of Chemistry, CA, USA. Title: "Chemoprevention of photocarcinogenesis by small molecules from plants". (March 12, 2014).
  • International Conference on 'Vistas of Life Sciences- Now and Beyond' at Jawaharlal Nehru University, New Delhi, India. Title: "Angioprevention of cancer". (February 13-15, 2014).
  • South Asian University, New Delhi, India. Title: "Starvation of tumor - chemopreventive intervention of angiogenic switch to control cancer". (Jan 24, 2014)
  • Fourth International Conference on Stem Cells and Cancer: Proliferation, Differentiation & Apoptosis" at Haffkine Institute, Parel, Mumbai, India. Chaired a scientific session on 'Molecular regulation in cancer therapeutics'. Title: Chemopreventive agents target tumor angiogenesis. (October 19-22, 2013)
  • DBT-Brain Storming Session on Nutriepigenomics at CFTRI Mysore, India. Title: "Modulation of tumor suppressors by IP6 in prostate cancer". (June 7, 2013)
  • A National Seminar on "Role of Ayurveda in Rheumatoid Arthritis & Cancer" Organized by GR Ayurved University, Hoshiarpur, Punjab, India. Title: "Cancer chemoprevention by phytochemicals". (April 20-21, 2013)
  • A Conference on "Emerging Trends and Challenges in Basic and Translational Research in Biochemistry" by Centre of Advanced Study in Zoology, Banaras Hindu University, Varanasi, India. Title: "Vascular targeting of cancer growth by phytochemicals". (February 4-5, 2013)
  • A Conference on "Applications of Natural Products for Human Health and Bioremediation of Pollutants" organized by Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India. Title: "Role of naturally occurring compounds in angioprevention of cancer". (March 22-23, 2013)
  • Thirty second Convention of Indian Association for Cancer Research on "Emerging Trends in Cancer research: Road to prevention & cure & International Symposium on: Infection and Cancer" Title: "Tumor Angiogenesis - a potential target in chemoprevention of cancer". (February 13-16, 2013).
  • International Congress on Ayurvedic Concepts and Treatment of Malignant Disorders at Sri Dharmasthala Manjunatheshwara College of Ayurveda & Hospital, Udupi, Karnataka. Topic: Role of Phytochemicals in Chemoprevention of Cancer. (December 15-16, 2012)
  • XXXII Annual Conference of Society of Toxicology (India): New Paradigms in Toxicology (NPT-2012) at CSIR-Indian Institute of Toxicology Research, Lucknow, UP, India. Title: "Phytochemicals in angioprevention of cancer" (December 5-7, 2012).
  • Third International Conference on Stem Cells and Cancer (ICSCC-2012): Proliferation, Differentiation and Apoptosis at Ram Manohar Lohia Hospital, New Delhi, India. Topic: "Apoptotic mechanisms of silibinin in neoplastic cells". (October 27-30, 2012).
  • Fifth International Congress on Nutrition and Cancer at Elazig, Turkey. Topic: Decursin, a novel coumarin with anticancer activity in prostate cancer (September 09 - 11, 2012)
  • Indo-US collaborative Workshop focused on "Accelerating Botanicals Agent Development Research for Cancer Chemoprevention & Treatment", at Moffitt Cancer Center in Tampa, Florida, USA. Topic: "Multiple targets of phytochemicals in cancer chemoprevention - cell cycle, apoptosis and angiogenesis". (May 29-31, 2012).
  • International Conference on Mitochondria Research and Medicine and First Annual Meeting of the Society for Mitochondria Research and Medicine (SMRM-India), at Centre for Cellular and Biology (CCMB), Hyderabad, India. Topic: "Apoptotic mechanisms of silibinin in neoplastic cells". (December 9-10, 2011)
  • Inter-University Accelerator Centre, New Delhi. Ultraviolet radiation-induced molecular alterations and apoptotic signaling. (November 21-22, 2011)
  • International Conference on Natural Products and Cancer Targets: Progress and Promise, Zhengzhou University, Zhengzhou Province, China. Topic: "Decursin, a naturally occurring coumarin, suppresses various aspects of prostate cancer growth". (August 24-25, 2011)
  • International meeting and round table discussion involving American and Chinese Scientists on Translational Research for Cancer Control, at Institute of Medicinal Biotechnology, Chinese Academy of Medical Science, and Pekin Union Medical College, Beijing, China. Topic: "Translational research with phytochemicals for cancer control". (August 26-28, 2011)
  • International Conference on Carcinogenesis and Cancer Prevention organized by Carcinogenesis Foundation, USA, at DSI, Bangalore. Title: "Cancer chemopreventive activity of a naturally occurring novel coumarin compound". (February 16-18, 2011)
  • Three lectures on various principles and techniques on topics related to radiation, Central University of Bihar, Patna. (December, 2010)
  • International Cancer Research Symposium-2010 on Defining and Translating Science Behind the Disease at Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India. Title: "A coumarin compound inhibits prostate cancer cells growth via modulation of cell cycle progression". (December 20-22, 2010)
  • Word Congress on Cancer-2010, Kottayam, Kerala, India. Title: "Targeting cell cycle regulation in prostate cancer." (September 3-5, 2010)
  • University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA. Title: "Cyclin-dependent kinase inhibitors: role in chemoprevention of prostate cancer". (July 2, 2010)
  • Symposium on Natural Products in Health and Disease: Biochemical and Molecular Mechanisms. Punjab University Chandigarh, Punjab, India. Title: "Mechanisms of cancer chemoprevention by IP6". (March 5-6, 2010)
  • International Symposium on Current Status and Opportunities in Aromatic and Medicinal Plants. Central Institute for Medicinal and Aromatic Plants, Lucknow, UP, India. Title: "Role of phytochemicals in cancer control". (February 21-24, 2010)
  • International Symposium on Endocrinology and Reproduction: Molecular Mechanisms to Molecular Medicine and 28th Meeting of the Society for Reproductive Biology and Comparative Endocrinology. SCMM, JNU, New Delhi. Title: "Prostate tumor growth and progression and its chemoprevention". (February 4-6, 2010)
  • UGC Academic Staff College, Jawaharlal Nehru University, New Delhi, India. Title: "Chemoprevention of cancer". (January, 2010)
  • International Conference on Frontiers in Diagnosis, Prevention and Therapy of Cancer, Motilal Nehru Medical College, Allahabad, U.P., India. Title: Cell cycle regulation and carcinogenesis. (November 21, 2009)
  • Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India. Title: "Targeting angiogenesis by small molecules for cancer control". (October 30, 2009)
  • 13th ISCB International Conference on Interplay of Chemical and Biological Sciences: Impact on health and environment. University of Delhi, India. Title: Chemoprevention of prostate tumor growth and progression. (March 1, 2009)
  • Two lectures at UGC Academic Staff College, Jawaharlal Nehru University, New Delhi, India. Title: 'Cancer Chemoprevention' and 'Angioprevention of cancer'. (January 20 & 28, 2009)
  • Word Congress on Cancer-2009, Kottayam, Kerala, India. Title: "Antiangiogenic intervention of cancer by phytochemicals." (January 13, 2009)
  • Roswell Park Cancer Institute, Buffalo, New York, USA. Title: "Targeting tumor angiogenesis by phytochemicals for cancer control." (January 11, 2008)
  • Indo-US Symposium on Tobacco-related Diseases under 5th World Assembly on Tobacco Counter Health (WATCH) Conference, New Delhi, India. Title: "Chemoprevention of lung cancer by targeting tumor angiogenesis." (December 4, 2007)
  • Biospark-2007, SLS, Jawaharlal Nehru University, New Delhi, India. Title: "Vascular targeting for cancer control." (March 23, 2007)
  • Academic Staff College, Jawaharlal Nehru University, New Delhi, India. Title: "Anticancer drug development from phytochemicals." (February 21, 2007)
  • 75th Annual meeting of Society of Biological Chemists (India) at Jawaharlal Nehru University, New Delhi, India. Title: "Mitogenic and survival signaling - potential targets for non-toxic anticancer agents". (December 11, 2006)
  • International Conference on 'Molecular and Physiological Effect of Bioactive Food Components' organized by University of Vienna and Medical University of Vienna, at Vienna, Austria. Title: "Angioprevention - an upcoming approach in cancer intervention by bioactive flavonoids". (October 13, 2006)
  • Health Products and Food Branch, Office of Continuing Education, Health Canada, Ottawa, Canada. Title: "Bioactive phytochemicals in prevention of cancer". (September 6, 2006)
  • School of Medicine, University of Colorado, Prostate Conference Series. Title: "Signal transduction pathways: potential targets in prostate cancer prevention". (March 6, 2006)
  • National Institute of Immunology, New Delhi, India. Title: "A plant flavonoid silibinin targets cell cycle regulation, mitogenic and survival signaling in prostate cancer". (September 7, 2005)
  • School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. Title: "Mechanisms of skin cancer chemoprevention and therapy by silibinin". (September 6, 2005)
  • School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. Title: "Targeting cell cycle regulation, mitogenic and survival signaling in prostate cancer chemoprevention" (February 3, 2004)
  • Presentation and discussion in 93rd Annual meeting of American Association for Cancer Research, Philadelphia, PA, USA. "Prostate cancer, silibinin and IGFBP-3". (April, 2002)

Research Grants

  1. Project Title

    Study of Antiangiogenic and Anti-tumor Effects of Fisetin in Lung Cancer: Implication for Intervention of Cancer

    Funding Agency: ICMR, India

    Duration: 2012-2015

    Role: PI

  2. Project Title

    To study the role of protein glycosylation in cell-extracellular matrix interaction and cancer progression

    Funding Agency: UGC, India

    Duration: 2012-2015

    Role: PI

  3. Project Title

    Antiangiogenic effects and mechanisms of Acacetin- a potential role in cancer control

    Funding Agency: CSIR, India

    Duration: 2009-2012

    Role: PI

  4. Project Title

    Targeting cell signaling and cell cycle progression for the intervention of prostate cancer by decursin

    Funding Agency: DST, India

    Duration: 2008-2011

    Role: PI

Membership

  • Life member, Indian Association for Cancer Research.
  • Active member, American Association for Cancer Research.
  • Life member, Society for Reproductive Biology and Comparative Endocrinology.
  • Cancer Research and Care Academy, New Delhi.

Teaching

  • Two courses: (a) Molecular Cancer Biology; and (b) Radiation Biology, for M.Sc. at School of Life Sciences, Jawaharlal Nehru University (JNU), New Delhi, India (2006-)
  • Two courses: (a) Topics in Radiation and Cancer Biology; and (b) Theoretical Foundation for Experiments, for M.Phil./Ph.D. at School of Life Sciences, JNU, New Delhi, India (2006-)
  • Three courses for M.Phil./Ph.D. at School of Life Sciences, CUG, Gandhinagar, Gujarat, India (2010-)
  • One course: Methods in Molecular Toxicology, including Carcinogenesis for Ph.D. Students, at the Department of Pharmaceutical Sciences, University of Colorado Denver, USA (2003-2006)

Personnel Trained (Cancer research)

  • Over 40, from 2001-onwards, including Graduate Students, Research Scholars, including Research Assistants, Research Associates and Visiting Scientists
  • M.Phil. Degree awarded: 10 students (submitted 1)
  • Ph.D. Degree awarded: 2 students (submitted 1)
  • Students continuing for Ph.D. Degree: 6 (+12 CUG)

Editorial Board Member for Peer-reviewed Journals

  • Frontiers in Cellular Biochemistry - Review Editor (2013- )
  • BMC-Cancer - Associate Editor (2013- )
  • Molecular Cancer Biology - Editor (2012- )
  • PLoS One - Academic Editor (2011- )
  • BMC Complementary and Alternative Medicine - Associate Editor - Basic Research (2011-14 )
  • International Journal of BioSciences and Technology (2008-12)
  • International Journal of Medical Sciences and Technology (2008-12)
  • International Journal of Life Sciences and Technology (2008-11)
  • Oriental Pharmacy and Experimental Medicine - Guest Editor for one article (2008)
  • Journal of Cancer Research and Therapeutics (2006-08)

Guest Editor for Special Issues

  • Mitochondrion (May, 2014) -Role of Mitochondria in Health and Disease (Impact Factor = 4.1, 2013)
  • Nutrition and Cancer (May, 2013) - Mechanisms of Bioactive Phytochemicals in Cancer Chemoprevention (Impact Factor = 2.8)
  • Indian Journal of Experimental Biology (Nov. 2011) - Emerging Trends in Cancer: Prevention and Therapeutics (Impact Factor = 1.3)

Reviewer for Edited Book

Molecular Techniques in Biochemistry and Biotechnology, McGraw-Hill, 2008

Reviewer for Peer-reviewed Journals

(1) Cancer Research, (2) Clinical Cancer Research, (3) Oncogene, (4) Molecular Carcinogenesis, (5) International Journal of Cancer, (6) Cancer Letters, (7) Nutrition and Cancer, (8) Indian Journal of Cancer, (9) International Journal of Gynecological Cancer, (10) Journal of Cell Physiology, (11) Archives of Biochemistry and Biophysics, (12) Drugs, (13) Evidenced-based Complementary and Alternative Medicine, (14) IUBMB Life, (15) Journal of Food Biochemistry, (16) Kidney International, (17) Toxicology Letters, (18) Food and Chemical Toxicology, (19) International Journal of Integrative Biology, (20) Cancer Immunology and Immunotherapy, (21) Journal of Neurosurgery, (22) Journal of Medicinal Food, (23) PLOS One, (24) BMC Cancer Biology, (25) International Journal of Radiation Biology, (26) Tumor Biology, (27) Molecular Cancer Therapeutics, (28) Cancer Discovery, (29) British Journal of Nutrition.

Publications (2000 - 2014)

Summary - Total: 111 (Research articles: 103; Book chapters: 8)

Research Articles:

  • Nambiar D, Deep G, Singh RP, Agarwal C, Agarwal R. Silibinin inhibit aberrant lipid metabolism, proliferation and emergence of androgen-independence in prostate cancer cells via primarily targeting the sterol response element binding protein 1. Oncotarget, 5, 2014.
  • Tailor D, Hahm ER, Kale RK, Singh SV, Singh RP. Sodium butyrate induces DRP1-mediated mitochondrial fusion and apoptosis in human colorectal cancer cells. Mitochondrion, 16, 55-64, 2014.
  • Jhala DV, Kale RK, Singh RP. Microgravity alters cancer growth and progression. Current Cancer Drug Targets, 14(4):394-406, 2014.
  • Mittal A, Tabasum S, Singh RP. Berberine in combination with doxorubicin suppresses growth of murine melanoma B16F10 cells in culture and xenograft. Phytomedicine, 21, 340-47, 2014.
  • Vundru SS, Kale RK, Singh RP. β-sitosterol induces G1 arrest and causes depolarization of mitochondrial membrane potential in breast carcinoma MDA-MB-231 cells. BMC Complement Alternative Medicine, 13(1):280, 2013.
  • Agarwal R, Kale RK, Rao CV, Singh RP. Introduction to Special Issue on Molecular Basis for Cancer Prevention with Bioactive Food Components in Nutrition and Cancer--an International Journal. Nutrition and Cancer, 65 S1:1-2. 2013.
  • Bhat TA, Nambiar D, Tailor D, Pal A, Agarwal R, Singh RP. Acacetin inhibits in vitro and in vivo angiogenesis and down-regulates Stat signaling and VEGF expression. Cancer Prevention Research, 6, 1128-39, 2013.
  • Singh N, Nambiar D, Kale RK, Singh RP. Usnic Acid inhibits growth and induces cell cycle arrest and apoptosis in human lung carcinoma A549 cells. Nutrition and Cancer, 65 (S1), 36-43, 2013.
  • Nambiar D, Singh RP. Advances in prostate cancer chemoprevention: a translational perspective. Nutrition and Cancer, 65 (S1), 12-25, 2013.
  • Nambiar D, Prajapati V, Agarwal R, Singh RP. In vitro and in vivo anticancer efficacy of silibinin against human pancreatic cancer BxPC-3 and PANC-1 cells. Cancer Letters, 2012 Sep 26. doi: pii: S0304-3835(12)00539-3.
  • Bhat TA, Nambiar D, Pal A, Agarwal R, Singh RP. Fisetin inhibits various attributes of angiogenesis in vitro and in vivo: Implications for angioprevention. Carcinogenesis, 33; 385-93, 2012.
  • Tyagi A, Agarwal C, Dwyer-Nield LD, Singh RP, Malkinson AM, Agarwal R. Silibinin modulates TNF-α and IFN-γ mediated signaling to regulate COX2 and iNOS expression in tumorigenic mouse lung epithelial LM2 cells. Molecular Carcinogenesis, 51, 832-42, 2012.
  • Nambiar D, Rajamani P, Singh RP. Effects of phytochemicals on ionization radiation-mediated carcinogenesis and cancer therapy. Mutation Research Reviews, 728; 139-57, 2011.
  • Kaur M, Tyagi A, Singh RP, Sclafani RA, Agarwal R, Agarwal C. Grape seed extract upregulates p21 (Cip1) through redox-mediated activation of ERK1/2 and post-transcriptional regulation leading to cell cycle arrest in colon carcinoma HT29 cells. Molecular Carcinogenesis, 50; 553-62, 2011.
  • Ramasamy K, Dwyer-Nield LD, Serkova NJ, Hasebroock KM, Tyagi A, Raina K, Singh RP, Malkinson AM, Agarwal R. Silibinin prevents lung tumorigenesis in wild-type but not in iNOS-/- mice: potential of Real-Time Micro-CT in lung cancer chemoprevention studies. Clinical Cancer Research, 16; 753-61, 2011.
  • Bhat TA, Moon JS, Lee S, Yim DS, Singh RP. Inhibition of angiogenic attributes by decursin in endothelial cells and ex vivo rat aortic ring angiogenesis model. Ind. J. Experimental Biology, 49; 848-56, 2011.
  • Prajapati V, Kale RK, Singh RP. Arsenic and its combinations in cancer therapeutics. Therapeutic Delivery, 2; 793-806, 2011.
  • Ravichandran K, Velmurugan B, Gu M, Singh RP, Agarwal R. Inhibitory effect of silibinin against azoxymethane-induced colon tumorigenesis in A/J mice. Clinical Cancer Research, 16; 4595-606, 2010.
  • Rajamanickam S, Velmurugan B, Kaur M, Singh RP, Agarwal R. Chemoprevention of intestinal tumorigenesis in APCmin/+ mice by silibinin. Cancer Research, 70; 2368-78, 2010.
  • Velmurugan B, Singh RP, Agarwal R, Agarwal C. Dietary feeding of grape seed extract prevents azoxymethane-induced colonic aberrant crypt foci formation in Fischer 344 rats. Molecular Carcinogenesis, 49; 641-52, 2010.
  • Kaur M, Velmurugan B, Tyagi A, Agarwal C, Singh RP, Agarwal R. Silibinin suppresses growth of human colorectal carcinoma SW480 cells in culture and xenograft through down-regulation of beta-catenin-dependent signaling. Neoplasia, 12; 415-24, 2010.
  • Mateen S, Tyagi A, Agarwal C, Singh RP, Agarwal R. Silibinin inhibits human non-small cell lung cancer cell growth through cell-cycle arrest by modulating expression and function of key cell-cycle regulators. Molecular Carcinogenesis, 49; 247-58, 2010.
  • Velmurugan B, Singh RP, Kaul N, Agarwal R, Agarwal C. Dietary feeding of grape seed extract prevents intestinal tumorigenesis in APCmin/+ mice. Neoplasia, 12; 95-102, 2010.
  • Singh RP, Raina K, Deep G, Chan D, Agarwal R. Silibinin suppresses growth of human prostate carcinoma PC-3 orthotopic xenograft via activation of extracellular signal-regulated kinase 1/2 and inhibition of signal transducers and activators of transcription signaling. Clinical Cancer Research, 15; 613-21, 2009.
  • Rajamanickam S, Kaur M, Velmurugan B, Singh RP, Agarwal R. Silibinin Suppresses Spontaneous Tumorigenesis in APC (min/+) Mouse Model by Modulating Beta-Catenin Pathway. Pharmaceutical Research, 26; 2558-67, 2009.
  • Roy S, Gu M, Ramasamy K, Singh RP, Agarwal C, Siriwardana S, Sclafani RA, Agarwal R. p21/Cip1 and p27/Kip1 Are essential molecular targets of inositol hexaphosphate for its antitumor efficacy against prostate cancer. Cancer Research, 69; 1166-73, 2009.
  • Singh RP, Agarwal R. Cosmeceuticals and silibinin. Clinics in Dermatology, 27; 479-84, 2009.
  • Tyagi A, Singh RP, Ramasamy K, Raina K, Redente EF, Dwyer-Nield LD, Radcliffe RA, Malkinson AM, Agarwal R. Growth inhibition and regression of lung tumors by silibinin: modulation of angiogenesis by macrophage-associated cytokines and nuclear factor-kappaB and signal transducers and activators of transcription 3. Cancer Prevention Research, 2; 74-83, 2009.
  • Kang TJ, Lee SY, Singh RP, Agarwal R, Yim DS. Anti-tumor activity of oxypeucedanin from Ostericum koreanum against human prostate carcinoma DU145 cells. Acta Oncologica, 48; 895-900, 2009.
  • Singh RP, Gu M, Agarwal R. Silibinin inhibits in vivo growth of colorectal cancer by inhibiting tumor cell proliferation and angiogenesis. Cancer Research, 68; 2034-50, 2008.
  • Raina K, Rajamanickam S, Singh RP, Deep G, Chittezhath M, Agarwal R. Stage-specific inhibitory effects and associated mechanisms of silibinin on tumor progression and metastasis in transgenic adenocarcinoma of the mouse prostate model. Cancer Research, 68; 6822-30, 2008.
  • Singh RP, Raina K, Sharma G, Agarwal R. Silibinin inhibits established prostate tumor growth, progression, invasion, and metastasis and suppresses tumor angiogenesis and epithelial-mesenchymal transition in transgenic adenocarcinoma of the mouse prostate model mice. Clinical Cancer Research, 14; 7773-80, 2008.
  • Velmurugan B, Singh RP, Tyagi A, Agarwal R. Inhibition of azoxymethane-induced colonic aberrant crypt foci formation by silibinin in male Fisher 344 rats. Cancer Prevention Research, 1; 376-84, 2008.
  • Deep G, Raina K, Singh RP, Oberlies NH, Kroll DJ, Agarwal R. Isosilibinin inhibits advanced human prostate cancer growth in athymic nude mice: Comparison with silymarin and silibinin. International Journal of Cancer, 123; 2750-8, 2008.
  • Chittezhath M, Deep G, Singh RP, Agarwal C, Agarwal R. Silibinin inhibits cytokine-induced signaling cascades and down-regulates inducible nitric oxide synthase in human lung carcinoma A549 cells. Molecular Cancer Therapeutics, 7; 1817-26, 2008.
  • Roy S, Singh RP, Agarwal C, Siriwardana S, Sclafani R, Agarwal R. Downregulation of both p21/Cip1 and p27/Kip1 produces a more aggressive prostate cancer phenotype. Cell Cycle, 12; 1828-35, 2008.
  • Raina K, Rajamanickam S, Singh RP, Agarwal R. Chemopreventive efficacy of inositol hexaphosphate on prostate tumor growth and progression in TRAMP mice. Clinical Cancer Research, 14; 2177-84, 2008.
  • Kaur M*, Singh RP*, Gu M, Agarwal R, Agarwal C. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells via induction of cell cycle arrest and apoptosis, and an up-regulation of Cip1/p21. Clinical Cancer Research, 12; 6194-202, 2006. *share first co-author.
  • This article was selected for the 'Highlights' section of the journal issue.

    Singh RP , Deep G, Chittezhath M, Kaur M, Dwyer-Nield LD, Malkinson AM, Agarwal R. Effect of silibinin on the growth and progression of primary lung tumors in mice. Journal of National Cancer Institute, 98; 846-55, 2006.
  • In Brief, Silibinin and lung tumor growth and progression. JNCI, 98; 799, 2006.
  • Mallikarjuna GU, Singh RP , Dhanalakshmi S, Mohan S, Agarwal R. Differential Effects of silibinin on E2Fs and associated biological events in chronically ultraviolet B-exposed skin versus tumors in SKH1 hairless mice. Molecular Cancer Therapeutics, 5; 2121-29, 2006.
  • Singh RP , Agarwal R. Natural flavonoids targeting deregulated cell cycle progression in cancer cells. Cancer Drug Targets, 7; 345-54, 2006.
  • Tyagi A, Singh RP , Agarwal C, Agarwal R. Silibinin activates p53-caspase-2 pathway and causes caspase-mediated cleavage of cip1/p21 in apoptosis induction in bladder transitional-cell papilloma RT4 cells: an evidence for a regulatory loop between p53 and caspase-2. Carcinogenesis, 27; 2269-80, 2006.
  • Singh RP , Dhanalakshmi S, Mohan S, Agarwal C, Agarwal R. Silibinin inhibits UVB- and EGF-induced mitogenic and cell survival signaling involving AP-1 and NF-kappa B in mouse epidermal JB6 cells. Molecular Cancer Therapeutics, 5; 1145-53, 2006.
  • Veluri R, Singh RP, Liu Z, Thompson JA, Agarwal R, Agarwal C. Fractionation of grape seed extract and identification of gallic acid as one of the major active constituents causing growth inhibition and apoptotic death of DU145 human prostate carcinoma cells. Carcinogenesis, 27; 1445-53, 2006.
  • Deep G, Singh RP, Agarwal C, Kroll DJ, Agarwal R. Silymarin and silibinin cause G1 and G2-M cell cycle arrest via distinct circuitries in human prostate cancer PC3 cells: a comparison of flavanone silibinin with flavanolignan mixture silymarin. Oncogene, 25; 1053-69, 2006.
  • Singh RP, Banerjee S, Kumar PV, Raveesha KA, Rao AR. Tinospora cordifolia induces enzymes of carcinogen/drug metabolism and antioxidant system, and inhibits lipid peroxidation in mice. Phytomedicine, 1-2; 74-84, 2006.
  • Varghese L, Agarwal C, Tyagi A, Singh RP, Agarwal R. Silibinin efficacy against human hepatocellular carcinoma. Clinical Cancer Research, 11; 8841-48, 2005.
  • Tyagi A, Singh RP, Agarwal C, Siriwardana S, Sclafani RA, Agarwal R. Resveratrol causes Cdc2-tyr15 phosphorylation via ATM/ATR-Chk1/2-Cdc25C pathway as a central mechanism for S phase arrest in human ovarian carcinoma Ovcar-3 cells. Carcinogenesis, 26; 1978-87, 2005.
  • Gu M, Dhanalakshmi S, Singh RP, Agarwal R. Silibinin inhibits ultraviolet B radiation-induced mitogenic and survival signaling, and associated biological responses in SKH-1 mouse skin. Carcinogenesis, 26: 1404-13, 2005.
  • Dhanalakshmi S, Agarwal C, Singh RP, Agarwal R. Silibinin up-regulates DNA-PK-dependent p53 activation to enhance UVB-induced apoptosis in mouse epithelial JB6 cells. Journal of Biological Chemistry, 280: 20375-83, 2005.
  • Gu M, Dhanalakshmi S, Singh RP, Agarwal R. Dietary feeding of silibinin prevents early biomarkers of ultraviolet B radiation-induced carcinogenesis in SKH-1 hairless mouse epidermis. Cancer Epidemiology Biomarkers and Prevention, 14: 1344-9, 2005.
  • Singh RP, Agrawal P, Yim D, Agarwal C, Agarwal R. Acacetin inhibits cell growth and cell cycle progression, and induces apoptosis in human prostate cancer cells: structure-activity relationship with linarin and linarin acetate. Carcinogenesis, 26: 845-54, 2005.
  • Padmavathi B, Upreti M, Singh V, Rao AR, Singh RP, Rath PC. Chemoprevention by Hippophae rhamnoides: effects on tumorigenesis, phase II and antioxidant enzymes and IRF-1 transcription factor. Nutrition and Cancer, 51: 59-67, 2005.
  • Padmavathi B, Rath PC, Rao AR, Singh RP. Roots of Withania somnifera inhibit forestomach and skin carcinogenesis in mice. Evidenced-based Alternative and Complementary Medicine, 2: 99-105, 2005.
  • Yim D, Singh RP, Agarwal C, Lee S, Chi H, Agarwal R. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma DU145 cells. Cancer Research , 65: 1035-44, 2005.
  • Singh RP, Dhanalakshmi S, Agarwal C, Agarwal R. Silibinin strongly inhibits growth and survival of endothelial cells via cell cycle arrest and down-regulation of survivin, Akt and NF-kappa B: implications for angioprevention and antiangiogenic therapy. Oncogene, 24: 1188-202, 2005.
  • Kaur M, Agarwal C, Singh RP, Guan X, Dwivedi C, Agarwal R. Skin cancer chemopreventive agent, alpha-santalol, induces apoptotic death of human epidermoid carcinoma A431 cells via caspases activation together with dissipation of mitochondrial membrane potential and cytochrome c release. Carcinogenesis, 26: 369-80, 2005.
  • Singh RP, Agarwal R. Mechanisms and preclinical efficacy of silibinin in preventing skin cancer. European Journal of Cancer, 41: 1969-79, 2005 .
  • Singh RP, Agarwal R. Inositol hexaphosphate and prostate cancer: efficacy and mechanisms. Anticancer Research, 25: 2891-903, 2005.
  • Agarwal C, Dhanalakshmi S, Singh RP, Agarwal R. Inositol hexaphosphate inhibits growth, and induces G1 arrest and apoptotic death of androgen-dependent human prostate carcinoma LNCaP cells. Neoplasia, 6: 646-59, 2004.
  • Singh RP, Mallikarjuna GU, Sharma G, Dhanalakshmi S, Tyagi AK, Chan DC, Agarwal C, Agarwal R. Oral silibinin inhibits lung tumor growth in athymic nude mice and forms a novel chemo-combination with doxorubicin targeting NF-kappa B-mediated inducible chemoresistance. Clinical Cancer Research, 10: 8641-7, 2004.
  • Mallikarjuna G, Dhanalakshmi S, Singh RP, Agarwal C, Agarwal R. Silibinin protects from photocarcinogenesis via modulation of cell cycle regulators, and mitogen-activated protein kinases and Akt signaling. Cancer Research, 64: 6349-56, 2004.
  • Mohan S, Dhanalakshmi S, Mallikarjuna GU, Singh RP, Agarwal R. Silibinin modulates UVB-induced apoptosis via mitochondrial proteins, caspases activation and mitogen-activated protein kinase signaling in human epidermoid carcinoma A431 cells. Biochemical Biophysical Research Communications, 320: 183-9, 2004.
  • Singh RP, Agarwal R. Prostate cancer prevention by silibinin. Current Cancer Drug Target, 4: 1-11, 2004.
  • Singh RP, Agarwal R. A cancer chemopreventive agent silibinin, targets mitogenic and cell survival signaling in prostate cancer. Mutation Research, 555: 21-32, 2004.
  • Dhanalakshmi S, Mallikarjuna GU, Singh RP, Agarwal R. Silibinin prevents ultraviolet radiation-caused skin damages in SKH-1 hairless mice via a decrease in thymine dimer positive cells and an up-regulation of p53-p21/Cip1 in epidermis. Carcinogenesis, 25: 1459-65, 2004.
  • Sharma G, Tyagi AK, Singh RP, Chan DC, Agarwal R. Synergistic anticancer effects of grape seed extract and conventional cytotoxic agent doxorubicin against human breast carcinoma cells. Breast Cancer Research and Treatment, 85; 1-12, 2004.
  • Singh RP, Sharma G, Mallikarjuna GU, Dhanalakshmi S, Agarwal C, Agarwal R. In vivo suppression of hormone-refractory prostate cancer growth by inositol hexaphosphate: induction of insulin-like growth factor binding protein-3 and inhibition of vascular endothelial growth factor. Clinical Cancer Research, 10, 244-50, 2004.
  • Agarwal C, Singh RP, Dhanalakshmi S, Agarwal R. Antiangiogenic efficacy of grape seed extract in endothelial cells. Oncology Reports, 11: 681-685, 2004.
  • Singh RP, Tyagi AK, Dhanalakshmi S, Agarwal R, Agarwal C. Grape seed extract inhibits advanced human prostate tumor growth and angiogenesis and upregulates insulin-like growth factor binding protein-3. International Journal of Cancer, 108: 733-40, 2004.
  • Dhanalakshmi S, Mallikarjuna GU, Singh RP, Agarwal R. Dual-efficacy of silibinin in protecting or enhancing ultraviolet B radiation-caused apoptosis in human immortalized keratinocyte HaCaT cells. Carcinogenesis, 25: 99-106, 2004.
  • Agarwal C, Singh RP, Dhanalakshmi S, Tyagi AK, Tecklenburg M, Sclafani RA, Agarwal R. Silibinin up-regulates the expression of cyclin-dependent kinase inhibitors, and causes cell cycle arrest and apoptosis in human colon carcinoma HT-29 cells. Oncogene, 22: 8271-82, 2003.
  • Tyagi AK, Agarwal C, Singh RP, Shroyer KR, Glode LM, Agarwal R. Silibinin down-regulates survivin protein and mRNA expression and causes caspases activation and apoptosis in human bladder transitional-cell papilloma RT4 cells. Biochemical Biophysical Research Communications, 312: 1178-84, 2003.
  • Agarwal C, Dhanalakshmi S, Singh RP, Agarwal R. Inositol hexaphosphate inhibits constitutive activation of NF- kappa B in androgen-independent human prostate carcinoma DU145 cells. Anticancer Research, 23: 3855-61, 2003.
  • Qi L, Singh RP, Lu Y, Agarwal R, Harrison GS, Franzusoff A, Glode LM. Epidermal growth factor receptor mediates silibinin-induced cytotoxicity in a rat glioma cell line. Cancer Biology and Therapy, 2: 526-31, 2003.
  • This article was selected for the "commentary" by the journal: Jonathan A. F. Hannay and Dihua Yu. Silibinin, a thorny therapeutic for EGFR expressing tumors. Cancer Biology and Therapy, 2: 532-33, 2003.
  • Singh RP, Sharma G, Dhanalakshmi S, Agarwal C, Agarwal R. Suppression of advanced human prostate tumor growth in athymic mice by silibinin feeding is associated with reduced cell proliferation, increased apoptosis and inhibition of angiogenesis. Cancer Epidemiology Biomarkers and Prevention, 12: 933-9, 2003.
  • Sharma G, Singh RP, Agarwal R. Growth inhibitory and apoptotic effects of inositol hexaphosphate in transgenic adenocarcinoma of mouse prostate (TRAMP-C1) cells. International Journal of Oncology, 23: 1413-8, 2003.
  • Sharma G, Singh RP, Chan DC, Agarwal R. Silibinin induces growth inhibition and apoptotic cell death in human lung carcinoma cells. Anticancer Research, 23: 2649-56, 2003.
  • Singh RP, Agarwal C, Agarwal R. Inositol hexaphosphate inhibits growth, and induces G1 arrest and apoptotic death of prostate carcinoma DU145 cells: modulation of CDKI-CDK-cyclin and pRb-related protein-E2F complexes. Carcinogenesis, 24: 555-63, 2003.
  • Singh RP, Agarwal R. Tumor angiogenesis: a potential target in cancer control by phytochemicals. Current Cancer Drug Target, 3: 205-17, 2003.
  • Agarwal C, Singh RP, Agarwal R. Grape seed extract inhibits growth and induces apoptotic death of prostate carcinoma DU145 cells via caspases activation, accompanied by dissipation of mitochondrial membrane potential and cytochrome c release. Carcinogenesis, 23: 1869-76, 2002.
  • Singh RP, Agarwal R. Flavonoid antioxidant silymarin and skin cancer. Antioxidant and Redox Signaling, 4: 655-63, 2002.
  • Singh RP, Dhanalakshmi S, Agarwal R. Phytochemicals as cell cycle modulators: a less toxic approach in halting human cancers. Cell Cycle, 1: 156-61, 2002.
  • Tyagi AK, Singh RP, Agarwal C, Chan DC, Agarwal R. Silibinin strongly synergizes human prostate carcinoma DU145 cells to doxorubicin-induced growth inhibition, G2-M arrest and apoptosis. Clinical Cancer Research, 8: 3512-19, 2002.
  • This article was selected for the "commentary" by the journal: Robert S. DiPaola. To arrest or not to G2-M cell-cycle arrest. Clinical Cancer Research, 8: 3311-4, 2002.

  • Singh RP, Dhanalakshmi S, Tyagi AK, Chan DC, Agarwal C, Agarwal R. Dietary feeding of silibinin inhibits advance human prostate carcinoma growth in athymic nude mice, and increases plasma insulin-like growth factor-binding protein-3 levels. Cancer Research, 62: 3063-9, 2002.
  • Singh RP, Tyagi AK, Zhao J, Agarwal R. Silymarin inhibits growth and causes regression of established skin tumors in SENCAR mice via modulation of mitogen-activated protein kinases and induction of apoptosis. Carcinogenesis, 23: 499-510, 2002.
  • Dhanalakshmi S, Singh RP, Agarwal C, Agarwal R. Silibinin inhibits constitutive and TNF-alpha-induced activation of NF-kappa B and sensitizes human prostate carcinoma DU145 cells to TNFa-induced apoptosis. Oncogene, 21: 1759-67, 2002.
  • Singh RP, Banerjee S, Rao AR. Modulatory influence of Andrographis paniculata on mouse hepatic and extrahepatic carcinogen metabolizing enzymes and antioxidant status. Phytotherapy Research, 15: 382-90, 2001.
  • Zi X, Singh RP, Agarwal R. Impairment of erbB1 receptor and fluid-phase endocytosis, and associated mitogenic signaling by inositol hexaphosphate in human prostate carcinoma DU145 cells. Carcinogenesis, 21: 2225-35, 2000.
  • Singh RP, Padmavathi B, Rao AR. Chemomodulatory influence of Adhatoda vesica (Justicia adhatoda) on the enzymes of xenobiotic metabolism, antioxidant status and lipid peroxidation in mice. Molecular and Cellular Biochemistry, 213: 99-109, 2000.
  • Singh RP, Banerjee S, Rao AR. Effect of Aegle marmelos on biotransformation enzymes system and protection against free radicals mediated damage in mice. Journal of Pharmacy and Pharmacology, 52: 991-1000, 2000.
  • Singh RP , Dhanalakshmi S, Rao AR. Chemomodulatory action of Aloe vera on the profiles of enzymes associated with carcinogen metabolism and antioxidant status regulation in mice. Phytomedicine, 7: 209-19, 2000.
  • Book Chapters:

  • Singh N, Tailor D, Kale RK, Singh RP. Antioxidant phytochemicals in cancer chemoprevention. InPhytochemicals of Nutraceutical Importance and their Role in Human Health. Eds. Sharma G., Prakash D. CABI-UK publication, pp. 229-47, 2014.
  • Mohan V, Nambiar D, Kale RK, Singh RP. Cell death-inducing mechanisms of cancer chemopreventive agents. In, Mitochondria as target for phytochemicals in cancer prevention. Ed. D. Chandra. Springer Science, New York, 2013.
  • Kartha A, Kale RK, Singh RP. Current trends in diagnosis, prevention and treatment of cancer. In Recent Trend in Life Sciences. Eds. Fulekar MH, Kale RK. IK International, New Delhi, pp. 265-319, 2013.
  • Taylor D, Singh RP. Dietary and non-dietary phytochemicals in cancer control. In Nutrition, Diet and Cancer. Eds. R. Srivastava & S. Shankar, Springer, pp. 573-610, 2012.
  • Bhat TA, Mittal A, Singh RP. Prevention of angiogenesis and metastasis. In Chemoprevention of cancer and DNA damage by dietary factors. Eds. S. Knasmuller, D.M. DeMarini, I. Johnson, C. Gerhauser. Wiley-VCH VerlagGmbH & Co. KgaA, Weinheim. pp. 163-182, April 2009.
  • Mittal A, Singh RP. Anticancer and immunomodulatory properties of Tinospora. In Herbal drugs: Ethnomedicine to modern medicine. Ed. K.G. Ramawat. Springer-Verlag Berlin Heidelberg, pp. 195-204, Dec, 2008.
  • Velmurugan B, Agarwal R, Singh RP. Role of bioactive natural compounds in cancer control. In Recent progresses in medicinal plant (phytopharmacology & therapeutic value III). Eds. J.N. Govil & V.K. Singh. Studium Press, LLC, Houston, TX, USA, vol. 21, pp. 367-93, Jan 2008.
  • Singh RP, Agarwal R. SENCAR mouse skin tumorigenesis model. In: Tumor models in cancer research. Ed. Beverly A. Teicher. Humana Press Inc. Totowa, NJ, USA, pp. 359-380, 2002.
  • Research Articles selected for Commentary/Highlights:

  • Singh RP, Raina K, Sharma G, Agarwal R. Silibinin inhibits established prostate tumor growth, progression, invasion, and metastasis and suppresses tumor angiogenesis and epithelial-mesenchymal transition in transgenic adenocarcinoma of the mouse prostate model mice. Clinical Cancer Research, 14; 7773-80, 2008.
  • Commentary - CCR Translations by Rupal S. Bhatt and Glenn J. Bubley. The challenge of herbal therapies for prostate cancer. Clinical Cancer Research, 14; 7581- 7582, 2008.
  • Kaur M*, Singh RP * , Gu M, Agarwal R, Agarwal C. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells via induction of cell cycle arrest and apoptosis, and an up-regulation of Cip1/p21. Clinical Cancer Research, 12; 6194- 202, 2006. *share first co-author.
  • This article was selected for the 'Highlights' section of the journal issue.

  • Qi L, Singh RP, Lu Y, Agarwal R, Harrison GS, Franzusoff A, Glode LM. Epidermal growth factor receptor mediates silibinin-induced cytotoxicity in a rat glioma cell line. Cancer Biology and Therapy, 2: 526-31, 2003.
  • Commentary by Jonathan A. F. Hannay and Dihua Yu. Silibinin, a thorny therapeutic for EGFR expressing tumors. Cancer Biology and Therapy, 2: 532-33, 2003.
  • Tyagi AK, Singh RP, Agarwal C, Chan DC, Agarwal R. Silibinin strongly synergizes human prostate carcinoma DU145 cells to doxorubicin-induced growth inhibition, G2-M arrest and apoptosis. Clinical Cancer Research, 8: 3512-19, 2002.
  • Commentary by Robert S. DiPaola. To arrest or not to G2-M cell-cycle arrest. Clinical Cancer Research, 8: 3311-4, 2002.
  • Articles in Conference Proceedings:

  • Nambiar D., Singh R.P. Anti-angiogenic intervention of cancer by chemopreventive phytochemicals. Proceeding of National Seminar on Role of Ayurveda in Rheumatoid Arthritis & Cancer Organized by GR Ayurved University, Hoshiarpur, Punjab, India (April 20-21, 2013)
  • Kumar NB, Dhurandhar M, Aggarwal BB, Anant S, Daniel K, Deng G, Djeu J, Dou J, Hawk E, Jayaram B, Jia L, Joshi R, Kararala M, Karunagaran D, Kucuk O, Kumar L, Malafa M, Samathanam GJ, Sarkar F, Siddiqi M, Singh RP, Srivastava A & White JD. Proceedings of the Indo-U.S. bilateral workshop on accelerating botanicals/ biologics agent development research for cancer chemoprevention, treatment, and survival. Cancer Medicine, 2(1); 108-115, 2013.
  • Paper/Abstract Presentation in National and International Conferences

  • Dhanya K Nambiar, Paul Rajamani, Rana P. Singh. Silibinin radiosensitizes prostate cancer cells by enhancing radiation-induced cell death and inhibiting nuclear EGFR-mediated DNA repair. Annual Meeting of AACR, 2014, San Diego, CA, USA. (April 5-9, 2014).
  • Dhanya K. Nambiar, Gagan Deep, Rana P. Singh, Chapla Agarwal, Rajesh Agarwal. Silibinin inhibits lipid metabolism by primarily targeting the master regulator sterol response element binding protein 1 (SREBP1) in prostate cancer cells. Annual Meeting of AACR-2014, San Diego, CA, USA. (April 5-9, 2014).
  • Dhanir Tailor, Eun-Ryeong Hahm, Raosaheb K. Kale, Shivendra V. Singh and Rana P. Singh. Title: Sodium butyrate, a product of microbial commensalism induces mitochondrial fusion and apoptosis in human colorectal cancer cells. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (Oral presentation) (November 19-20, 2013).
  • Nupoor Prasad, Dhanir Tailor and Rana P. Singh. Title: Study of anticancer efficacy of lupeol against cervical carcinoma HeLa cells. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Prerna Ramteke, Dhanir Tailor and Rana P. Singh. Title: Study of the effect of hesperetin on inflammatory mechanisms in lung cancer cells. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Reenu Punia, R. K. Kale, Man Singh and Rana P. Singh. Title: Physicochemical characterization of cancer chemopreventive agent, silibinin in complex with phosphatidylcholine International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Narendra Singh, Raosaheb K. Kale and Rana P. Singh. Title: Effect of usnic acid on growth and cell cycle progression of human lung carcinoma A549 cells. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Shantishree Vundru, Raosaheb K. Kale and Rana P. Singh. Title: The anti-proliferative and pro-apoptotic effects of β-sitosterol on human breast carcinoma cells. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Vijay Mohan and Rana P. Singh. Title: A novel anticancer agent, evodiamine, induces G2-M arrest in human lung carcinoma A549 cells. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Viral V. Surati, A. K. Singh and Rana P. Singh. Title: Antibacterial and phytochemical screening of Viola odorata. International Conference on Advances in Biotechnology and Bioinformatics at Dr. D. Y. Patil Vidyapeeth, Pune, India. (November 25-27, 2013).
  • Reenu Punia, R. K. Kale, Man Singh and Rana P. Singh. Title: Physicochemical characterization of silibinin in complex with carboxymethyl cellulose and implications for drug formulation. At International Conference on Advances in Biotechnology and Bioinformatics, Dr. D. Y. Patil Vidyapeeth, Pune, India. (November 25-27, 2013).
  • Vandana Prajapati, R.K. Kale, Rana P. Singh. Effects of silibinin against arsenic-induced skin cancer. Biosparks 2014, SLS, Jawaharlal Nehru University, New Delhi, India. (March 21-22, 2014). (Oral presentation)
  • Vandana Prajapati, R.K. Kale, Rana P. Singh. Chemopreventive effects of silibinin against arsenic-induced skin carcinogenesis in vitro and in vivo. XIII International Congress of Toxicology, Coex, Seoul, South Korea. (June 30 - July 4, 2013).
  • Sivapar V. Mathan and Rana P. Singh. Study of anticancer effects and associated mechanism of Diallyl trisulfide (DATS) on head and neck cancer. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Anil Mittal, Saba Tabassum and R. P. Singh. Berberine and Doxorubicin inhibits cancer cell growth in vitro and in vivo. International Conference on Recent Advances in Cancer Prevention and Therapeutics at Central University of Gujarat, Gandhinagar, India. (November 19-20, 2013).
  • Praveen Kumar Kujur, Rana P. Singh. Chemomodulation of liver cancer by Decursin. International Conference on Recent Advances in Cancer Research: Bench to Bedside, Gandhinagar, Gujarat, 19-20 February 2011.
  • Vandana Prajapati, R.K. Kale, Rana P. Singh. Modulatory effect of silibinin on arsenic-induced changes in cancer cells. 36th Annual Conference of Environmental Mutagen Society of India (EMSI) and International Symposium on Environmental Exposures to Mutagens and Carcinogens on Human Health, Vellore, Tamil Nadu, 4-6 February 2011.
  • Anil Mittal, Rana P. Singh. Combinatorial effects of berberine and doxorubicin on prostate cancer cells. International Conference on Recent Advances in Cancer Research: Bench to Bedside, Gandhinagar, Gujarat, 19-20 February 2011.
  • Dhanya K. Nambiar, Rana P. Singh. Radiosensitizing effects of silibinin in prostate cancer. International Cancer Research Symposium-2010 on Defining and Translating Science Behind the Disease, RGCB, Trivendrum, Kerala, 20-22 December, 2010.
  • Rana P. Singh, Tariq A. Bhat, Dhanya Nambiar, Velmurugan Balaiya, Rajesh Agarwal. Decursin inhibits advanced human prostate tumor xenograft growth involving antiproliferative and antiangiogenic mechanisms. 101st Annual Meeting of AACR, Washington, DC, USA, April 2010. (Late breaking)
  • Dhanya K. Nambiar, M. Chamoli, Rana P. Singh. In vitro assessment of combinatorial effect of silibinin and ionizing radiation on prostate cancer cells. International Symposium on Cancer Chemoprevention and Translational Research, Jawaharlal Nehru University, New Delhi, India, 21 December 2009.
  • Tariq A. Bhat, A. Pal, R. Agarwal and Rana P. Singh. Fisetin - a potential antiangiogenic phytochemical. International Symposium on Novel Strategies for Targeted Prevention and Treatment of Cancer, Jawaharlal Nehru University, New Delhi, India, 19-20 December 2008.
  • Rana P. Singh, Komal Raina, Gagan Deep and Rajesh Agarwal. Silibinin suppresses growth of human prostate carcinoma PC-3 orthotopic xenograft via inhibition of STAT and activation of ERK1/2 signaling. 99th Annual Meeting of AACR, San Diego, CA, USA, April 2008. (Late breaking)
  • Alpna Tyagi, Rana P. Singh, Kumaraguruparan Ramasamy, Lori D. Nield, Al Malkinson and Rajesh Agarwal. Growth inhibition of urethane-induced lung tumors in A/J mice by silibinin: modulation of angiogenesis by macrophage-associated cytokines, and STAT3 and NF-kB. 99th Annual Meeting of AACR, San Diego, CA, USA, April 2008.
  • K. Ramasamy, Alpna Tyagi, Elizabeth Redente, Rana P. Singh, Lori D. Nield, Al Malkinson and Rajesh Agarwal. Chemopreventive effect of silibinin on early lesions in urethane-induced lung tumorigenesis in A/J mice. 99th Annual Meeting of AACR, San Diego, CA, USA, April 2008.
  • Chapla Agarwal, Velmurugan Balaiya, Rana P. Singh, Nidhi Kaul and Rajesh Agarwal. Grape seed extract inhibits intestinal tumorigenesis in APCmin mice and decreases COX-2 and iNOS together with an increase in Cip1/p21 level. 99th Annual Meeting of AACR, San Diego, CA, USA, April 2008.
  • Velmurugan Balaiya, Rana P. Singh, Rajesh Agarwal and Chapla Agarwal. Inhibition of azoxymethane-induced colonic aberrant crypt foci formation by grape seed extract in male Fisher 344 rats. 99th Annual Meeting of AACR, San Diego, CA, USA, April 2008.
  • Rana P. Singh, Gagan Deep and Rajesh Agarwal. Silibinin inhibits growth of PC-3 prostate tumor xenograft involving up-regulation of cyclin-dependent kinase inhibitors and IGFBP-3, and down-regulation of surviving. Innovative Minds in Prostate Cancer Today (IMPACT), Department of Defense Prostate Cancer Research Program, Atlanta, Georgia, USA, 5-8 September 2007.
  • Rana P. Singh, Gagan Deep and Rajesh Agarwal. Dietary feeding of silibinin inhibits PC-3 tumor xenograft growth via ERK1/2 activation and an increase in Cip1/p21 and Kip1/p27 expression. 98th Annual Meeting of AACR, Los Angeles, CA, April 2007.
  • Rana P. Singh. Chemoprevention of lung cancer by targeting tumor angiogenesis. Indo-US Symposium on Tobacco-related Diseases under 5 th World Assembly on Tobacco Counter Health (WATCH) Conference, New Delhi, India, December 4, 2007.
  • Mallikarjuna Gu, Rana P. Singh and Rajesh Agarwal. Silibinin inhibits in vivo growth of colorectal cancer by inhibiting tumor cell proliferation and angiogenesis in athymic nude mice. 98th Annual Meeting of AACR, Los Angeles, CA, April 2007.
  • Dwyer-Nield LD, Rana P. Singh, Tyagi A, Malkinson AM and Agarwal R. Therapeutic efficacy of silibinin on chemically-induced mouse lung tumors. 2007 AACR Annual Meeting, Los Angeles, CA, April 14-18, 2007.
  • Alpna Tyagi, Rana P. Singh, Chapla Agarwal, Gail Harrison, LM Glode and Rajesh Agarwal. Chemopreventive effect of silibinin and silymarin on n-butyl-n-(4-hydroxybutyl) nitrosamine-induced urinary bladder carcinogenesis in male ICR mice. 98th Annual Meeting of AACR, Los Angeles, CA, April 2007.
  • Balaiya Velmurugan, Rana P. Singh, Alpna Tyagi and Rajesh Agarwal. Inhibition of azoxymethane-induced colonic aberrant crypt foci formation by silibinin in male Fisher 344 rats. 98th Annual Meeting of AACR, Los Angeles, CA, April 2007.
  • Rana P. Singh, Girish Sharma and Rajesh Agarwal. Silibinin consumption inhibits prostate tumor growth in transgenic adenocarcinoma of mouse prostate (TRAMP) mice. 97th Annual Meeting of AACR, Washington, DC, April 2006.
  • Gu Mallikarjuna, Rana P. Singh, S. Dhanalakshmi, Chapla Agarwal and Rajesh Agarwal. Inositol hexaphosphate down-regulates both constitutive and ligand-induced mitogenic and cell survival signaling, and causes caspase-mediated apoptotic death of human prostate carcinoma PC3 cells. 97th Annual Meeting of AACR, Washington, DC, April 2006.
  • Gu Mallikarjuna, S. Dhanalakshmi, Rana P. Singh, Chapla Agarwal and Rajesh Agarwal. Silibinin inhibits UVB-induced skin tumorigenesis by down-regulating STAT3 and NF-kappa B activation. 97th Annual Meeting of AACR, Washington, DC, April 2006.
  • Gagan Deep, Rana P. Singh, Chapla Agarwal, David J. Kroll and Rajesh Agarwal. Distinct efficacy of silibinin in causing G1 and G2-M arrests via cyclins-CDK-CDK inhibitors and Chk2-Cdc25C-Cdc2-cyclin B1 circuitries in human prostate cancer PC3 cells: comparison with crude form silymarin. 96th Annual Meeting of AACR, Anaheim, CA, USA. April 2005.
  • Gu Mallikarjuna, Sarumathi Mohan, S. Dhanalakshmi, Rana P. Singh and Rajesh Agarwal. Protective efficacy and associated mechanisms of silibinin against UVB-induced skin tumorigenesis in SKH-1 hairless mice. 96th Annual Meeting of AACR, Anaheim, CA, USA. April 2005.
  • Manesh Chittezhath, Rana P. Singh, Chapla Agarwal and Rajesh Agarwal. Silibinin inhibits cytokines-induced STATs, MAPKs and NF-kappaB activation and down regulates HIF-1-alpha and iNOS in human lung epithelial A549 cells: possible role in angioprevention of lung tumorigenesis. 96th Annual Meeting of AACR, Anaheim, CA, USA. April 2005.
  • Rajesh Agarwal, Gu Mallikarjuna, S. Dhanalakshmi, Sarumathi Mohan and Rana P. Singh. Silibinin inhibits ultraviolet B radiation-induced mitogenic and survival signaling, and associated proliferation and apoptosis in SKH-1 mouse skin: implications for the prevention of photocarcinogenesis. 96th Annual Meeting of AACR, Anaheim, CA, USA. April 2005.
  • Rana P. Singh, Leyon Varghese, S. Dhanalakshmi and Rajesh Agarwal. In vitro as well as in vivo anticancer efficacy of silibinin against human pancreatic cancer BxPC-3 and Panc-1 cells. 96th Annual Meeting of AACR, Anaheim, CA, USA. April 2005.
  • Sarumathi Mohan, S. Dhanalakshmi, Rana P. Singh, Chapla Agarwal and Rajesh Agarwal. Silibinin inhibits UVB- and EGF-induced mitogenic MAPK-AP1 and cell survival Akt-NF-kB signaling in mouse epidermal JB6 cells: Possible role in protection against skin tumor promotion. 96th Annual Meeting of AACR, Anaheim, CA, USA. April 2005.
  • Srirupa Roy, Manjinder Kaur, Chapla Agarwal, Rana P. Singh, Rajesh Agarwal. Role of cyclin-dependent kinase inhibitors p21 and p27 in silibinin-induced G1 arrest in DU145 prostate cancer cells. 96th Annual Meeting of AACR, Anaheim, CA, USA. April 2005.
  • Rana P. Singh and Rajesh Agarwal. Silibinin inhibits various attributes of angiogenesis: implications for angioprevention and antiangiogenic therapy. 23rd Annual Meeting of IACR, ACTREC, Mumbai, India. Jan 2004.
  • D. S. Yim, Rana P. Singh, Sook Y. Lee, Chapla Agarwal and Rajesh Agarwal. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma DU145 cells. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004.
  • Rana P. Singh, Girish Sharma, S. Dhanalakshmi, L. M. Glode and Rajesh Agarwal. Silibinin inhibits human bladder transitional-cell papilloma RT4 tumor xenograft growth in athymic nude mice. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004.
  • S. Dhanalakshmi, Gu Mallikarjuna, Rana P. Singh and Rajesh Agarwal.
  • Silibinin inhibits ultraviolet B-caused skin damages via up-regulation of p53 and p21/cip1 in SKH-1 hairless mice. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004 (selected for Minisymposium).
  • Gu Mallikarjuna, S. Dhanalakshmi, Rana P. Singh, C. Agarwal, Rajesh Agarwal. Protective effect of silibinin against photocarcinogenesis in SKH-1 hairless mice. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004.
  • Rana P. Singh, Gu Mallikarjuna, Girish Sharma, S. Dhanalakshmi, A. K. Tyagi, Daniel Chan, C. Agarwal and Rajesh Agarwal. Silibinin reduces toxicity and enhances therapeutic efficacy of doxorubicin in lung cancer. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004.
  • Manjinder Kaur, C. Agarwal, Rana P. Singh, Chandradhar Dwivedi and Rajesh Agarwal. Alpha-santalol induces apoptotic cell death in human epidermoid cancer cell line. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004.
  • Rana P. Singh, Puja Agarwal, Dong-Sool Yim and Rajesh Agarwal. Growth inhibitory and apoptotic effects of linarin, linarin acetate and acacetin on human prostate carcinoma cells. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004.
  • S. Dhanalakshmi, Sarumathi Mohan, Rana P. Singh, G. U. Mallikarjuna and Rajesh Agarwal. Silibinin enhances UVB-induced apoptosis in human epidermoid carcinoma A431 cells via proapoptotic mitochondrial proteins and caspases activation. 95th Annual Meeting of AACR, Orlando, FL, USA. March 2004.
  • Rana P. Singh, Girish Sharma, Gu Mallikarjuna, S. Dhanalakshmi, Chapla Agarwal and Rajesh Agarwal. Inositol hexaphosphate inhibits advanced human prostate tumor growth and angiogenesis in athymic nude mice. 94th Annual Meeting of AACR, Toronto, Canada. April 2003.
  • Chapla Agarwal, S. Dhanalakshmi, Rana P. Singh and Rajesh Agarwal. Antiangiogenic effect of grape seed extract in human prostate cancer xenograft. 94th Annual Meeting of AACR, Toronto, Canada. April 2003. (selected for Minisymposium).
  • A. K. Tyagi, Rana P. Singh, Chapla Agarwal, Kenneth Shroyer, L. Michael Glode and Rajesh Agarwal. Silibinin down-regulates surviving mRNA and protein expression accompanied by G2-M arrest and apoptosis in human bladder transitional-cell papilloma TR4 cells. 94th Annual Meeting of AACR, Toronto, Canada. April 2003. (selected for Minisymposium).
  • Rana P. Singh and Rajesh Agarwal. Silibinin inhibits growth and causes apoptotic death of endothelial cells via modulation of survivin and mitochondrial apoptosis. New Directions in Angiogenesis Research, special scientific conference of AACR at Chicago, IL, USA. October 2003.
  • Rana P. Singh, S. Dhanalakshmi and A. Ramesha Rao. Clerodendrum inerme up-regulates phase I & II enzymes, reduced glutathione and superoxide dismutase-catalase system in mice. International Conference on Emerging Trends in Cancer Research, SLS, Jawaharlal Nehru University, New Delhi, India. March 2002.
  • Chapla Agarwal, Rana P. Singh and Rajesh Agarwal. Grape seed extract-induced apoptotic death of prostate carcinoma DU145 cells: Involvement of caspases activation via cytochrome c release. 93rd Annual Meeting of AACR, San Francisco, CA, USA. April 2002.
  • S. Dhanalakshmi, Rana P. Singh, Chapla Agarwal and Rajesh Agarwal. Silibinin inhibits constitutive and TNFa-induced activation of NF-kB and sensitizes human prostate carcinoma DU145 cells to TNFa-induced apoptosis. 93rd Annual Meeting of AACR, San Francisco, CA, USA. April 2002.
  • Lin Qi, Rana P. Singh, Rajesh Agarwal and L. Michael Glode. The EGF receptor is necessary and sufficient to mediate silibinin-mediated cytotoxicity in transfected 9L glioma cells. American Society of Clinical Oncology, Molecular Therapeutics Symposium (MTS), San Diego, CA, USA. November 8-10, 2002.
  • Rana P. Singh, S. Dhanalakshmi, Anil K. Tyagi, Daniel C.F. Chan, Chapla Agarwal and Rajesh Agarwal. Dietary feeding of silibinin inhibits advance human prostate carcinoma growth in athymic nude mice, and increases plasma IGFBP-3 levels. 93rd Annual Meeting of AACR, San Francisco, CA, USA. April 2002. (Selected for Poster-Discussion Session).
  • Chapla Agarwal, Rana P. Singh, Anil K. Tyagi and Rajesh Agarwal. Anti-carcinogenic effect of grape seed extract against prostate cancer. 92nd Annual Meeting of American Association for Cancer Research, New Orleans, LA, USA. March 2001.
  • Rana P. Singh, Chapla Agarwal and Rajesh Agarwal. Novel anti-carcinogenic function of inositol hexaphosphate in human prostate carcinoma DU145 cells: Modulation of cell-cycle regulators, growth inhibition, and induction of G1 arrest. 92nd Annual Meeting of American Association for Cancer Research, New Orleans, LA, USA. March 2001.
  • Rana P. Singh, S. Banerjee and A. Ramesha Rao. Cancer chemopreventive potential of Andrographis paniculata in murine model system. 18th Annual convention of IACR and National Symposium on Molecular Biology of Cancer, All India Institute of Medical Sciences, New Delhi, India. February 1999.
  • Rana P. Singh, B. Padmavathi, S. Banerjee and A. Ramesha Rao. Chemomodulatory influence of Adhatoda vesica on carcinogen metabolizing enzymes and antioxidant mechanisms in murine model system. 18th Annual Convention of Indian Association for Cancer Research and National Symposium on Molecular Biology of Cancer, New Delhi, India. February 1999.
  • Rana P. Singh, S. Banerjee, A. Raveesha and A. Ramesha. Rao. Cancer chemopreventive potential of Tinospora cordifolia in murine model system. 67th Annual Meeting of Society of Biological Chemists (India), New Delhi, India. December 1998.